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The Characterization of Shiga Toxin‐Non‐Producing Escherichia coli Serotype O157:H7 Isolated from Carcasses of Cattle at a Slaughter House
Author(s) -
Itoh Yoshihiro,
Hayashi Naoko,
Katoh Mikio,
Yamamoto Atuko,
Hayashi Seizou,
Maeda Shigeki,
Ezaki Takayuki
Publication year - 1999
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1999.tb02458.x
Subject(s) - escherichia coli , biology , serotype , pulsed field gel electrophoresis , shiga toxin , microbiology and biotechnology , shiga like toxin , hemolysin , plasmid , toxin , genotype , enterobacteriaceae , veterinary medicine , gene , virulence , genetics , medicine
A bacteriological investigation of Shiga toxin (Stx)‐producing Escherichia coli (STEC) 0157:H7 was performed on 298 carcasses of cattle at slaughter houses between July 1996 and January 1997 in Gifu Prefecture, Japan. As a result, four Stx‐non‐producing Escherichia coli O157:H7 strains were isolated from two slaughtered carcasses of cattle. The purpose of this study was to examine the characterization of isolates. Isolates possessed the E. coli attaching and effacing gene ( eaeA ), and hemolysin gene ( hlyA ), and harbored 3.0‐MDa and 60‐MDa plasmids. The Xba I pulsed‐field gel electrophoresis (PFGE) pattern showed three similar patterns. Consequently, a closely related genotype of Stx‐non‐producing E. coli O157:H7 may widely exist in cattle.