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A Single Cell Analysis of TCR AV24AJ18 + DN T Cells
Author(s) -
Keino Hiroshi,
Matsumoto Isao,
Okada Seiji,
Kurokawa Manae,
Kato Tomohiro,
Tokuhisa Takeshi,
Usui Masahiko,
Taniguchi Masaru,
Nishioka Kusuki,
Sumida Takayuki
Publication year - 1999
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1999.tb02444.x
Subject(s) - t cell receptor , biology , microbiology and biotechnology , t cell , clone (java method) , epitope , antigen , cell sorting , natural killer t cell , antigen presenting cell , t lymphocyte , cytotoxic t cell , flow cytometry , gene , immunology , immune system , biochemistry , in vitro
The T‐cell receptor (TCR) BV gene of human TCR AV24 + double‐negative (DN) T cells, a novel subset of natural killer (NK) T cells, was investigated by single‐cell sorting and single‐cell polymerase chain reaction (PCR) methods. Seven of eleven TCR AV24 + DN T‐cell clones utilized TCR BV8, three BY9, and one BV6. Six of seven TCR AV24/BV8+ DN T‐cell clones had identical TCR β and α chains, indicating that they were the same clone. All three TCR AV24/BV9 + DN T‐cell clones also demonstrated the same amino acids in the CDR3 region. These findings strongly suggest that the usage of TCR β and α chains on TCR AV24 + DN T cells is extremely restricted, supporting the notion that these cells recognize highly limited T‐cell epitopes on antigens. All TCR AV24 + clones expressed the NKR‐P1A mRNA, and so were true NK T cells. IL‐2 and IL‐4 mRNAs were detected in all clones, suggesting that the majority of these cells were Th0‐type T cells. Six clones overexpressed Fas‐ligand (Fas‐L) mRNA and Fas antigen was detected on all clones at the mRNA level. In conclusion, TCR AV24 + DN T cells might recognize restricted T‐cell epitopes on antigens and function as Th0‐type T cells, inducer cells to Th1‐ or Th2‐type T cells (regulatory T cells), and as Fas‐L‐positive cytolytic T cells.