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A Filamentous Phage of Vibrio parahaemolyticus O3:K6 Isolated in Laos
Author(s) -
Nakasone Noboru,
Ikema Masahide,
Higa Naomi,
Yamashiro Tetsu,
Iwanaga Masaaki
Publication year - 1999
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1999.tb02420.x
Subject(s) - vibrio parahaemolyticus , biology , microbiology and biotechnology , pilus , serotype , dna , polyacrylamide gel electrophoresis , strain (injury) , vibrio cholerae , gel electrophoresis , bacteria , escherichia coli , biochemistry , gene , enzyme , genetics , anatomy
A filamentous phage, ‘lvpf5’, of Vibrio parahaemolyticus O3:K6 strain LVP5 was isolated and characterized. The host range was not restricted to serotype O3:K6, but 7 of 99 V. parahaemolyticus strains with a variety of serotypes were susceptible to the phage. The phage was inactivated by heating at 80 C for 10 min and by treating with chloroform. Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of the phage exhibited a 3.8 kDa protein. The amino‐terminal amino acid sequence of the coat protein was determined as AEGGAADPFEAIDLLGVATL. The phage genome consisted of a single‐stranded DNA molecule. The activity of the phages was inhibited by anti‐Na2 pili antibody.