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Molecular Epidemiological Study of a Mass Outbreak Caused by Enteropathogenic Escherichia coli O157:H45
Author(s) -
Makino Souichi,
Asakura Hiroshi,
Shirahata Toshikazu,
Ikeda Tetsuya,
Takeshi Koichi,
Arai Keisuke,
Nagasawa Motohiro,
Abe Tamotsu,
Sadamoto Terukazu
Publication year - 1999
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1999.tb02419.x
Subject(s) - outbreak , enteropathogenic escherichia coli , biology , escherichia coli , microbiology and biotechnology , virology , plasmid , vero cell , molecular epidemiology , toxin , gene , virus , genotype , genetics
We made a molecular analysis of O157:H45 Escherichia coli isolated from a mass outbreak that occurred in Obihiro City. Using DNA analysis, we confirmed this infection case as a mass outbreak. Although the isolates expressed O157 antigen, they did not produce Vero toxin. We concluded they were enteropathogenic E. coli (EPEC) because they had a bfp gene and an EAF plasmid, and further they exhibited local adherence to HEp‐2 cells. We believe this is the first report of a mass outbreak by O157 EPEC, and we suggest that PCR using eae ‐ and bfp ‐specific primers and HEp‐2 adherence assay are useful to identify EPEC.