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PCR‐RFLP Analysis of Cytomegalovirus Infections Associated with Bone Marrow Transplantation in Japanese Children
Author(s) -
Kawasaki Satoshi,
Oshitani Hitoshi,
Suzuki Hiroshi,
Arakawa Masaaki,
Mizuta Katsumi,
Imaizumi Masue,
Tsuchiya Shigeru,
Konno Tasuke
Publication year - 1999
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1999.tb02416.x
Subject(s) - biology , restriction fragment length polymorphism , genotype , cytomegalovirus , polymerase chain reaction , betaherpesvirinae , virology , transplantation , restriction enzyme , herpesviridae , human cytomegalovirus , immunology , viral disease , genetics , dna , virus , medicine , gene
In order to investigate the longitudinal molecular epidemiology of cytomegalovirus (CMV) infections associated with bone marrow transplantation (BMT) in Japanese children, we analyzed 36 CMV strains from 11 cases. Three regions (DNA polymerase, glycoprotein H, and immediate‐early regions) of CMV DNA were amplified by polymerase chain reaction (PCR), and amplified products were each digested with two restriction enzymes, followed by electrophoresis. These restriction fragment length polymorphism (RFLP) analyses allowed the differentiation of 36 strains into 13 genotypes. Each patient excreted his or her own CMV with distinct genotype over the study period of up to one year. CMVs of two different genotypes were recovered during a one‐month study from one recipient, who received a peripheral blood stem cell transplantation. Although the majority of patients and donors were CMV‐seropositive before BMT, multiple CMV infections might not be common and the reactivation of latently infected CMV might be prominent in Japanese children receiving transplants.