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Antibacterial Activities of New Synthetic Divalent Cation Chelators
Author(s) -
Ashoori Mandana,
Ohta Michio,
Ohsuka Shinji,
Shibayama Keigo,
Horii Toshinobu,
Ueda Minoru,
Kurosaki Hiromasa
Publication year - 1999
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1999.tb02410.x
Subject(s) - divalent , chelation , antibacterial activity , bacteria , periplasmic space , biochemistry , antibacterial agent , biology , chemistry , nuclear chemistry , escherichia coli , stereochemistry , antibiotics , organic chemistry , genetics , gene
A series of new synthetic ligand compounds which chelate divalent cations was examined for the antibacterial activities of the compounds. Only 2 of 14 synthetic chelators, 9‐ trans ‐anthryl‐1, 4, 8, 11‐tetraaza‐tetradecane (No. 6) and bis(2‐pyridyl)methylamine (No. 13) showed antibacterial activities, whereas none of the diamines, hydrophilic triamines nor tetramines showed antibacterial activities. Chelators No. 6 and No. 13 inhibited the growth of both Gram‐negative and ‐positive bacteria at doses of 25–200 μg/ml, comparable to those of common antibiotics such as polymixin B, fosfomycin and macrolides. Ethylenedi‐aminetetraacetate (EDTA) potentiated these antibacterial activities, whereas an inhibitory effect of Mg 2+ on the MICs of these chelators was observed. Moreover, these chelators enhanced the leakage of periplasmic β‐lactamase. It is therefore suggested that chelators No. 6 and No. 13 disrupt both the membranes and cytoplasmic functions of bacteria, resulting in cell death.