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Molecular Cloning and Characterization of the oprQ Gene Coding for Outer Membrane Protein OprE3 of Pseudomonas aeruginosa
Author(s) -
Okamoto Kiyomi,
Gotoh Naomasa,
Tsujimoto Hideto,
Yamada Hiroshi,
Yoshihara Eisaku,
Nakae Taiji,
Nishino Takeshi
Publication year - 1999
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1999.tb02407.x
Subject(s) - porin , biology , bacterial outer membrane , pseudomonas aeruginosa , gene , peptide sequence , transmembrane protein , sequence analysis , membrane protein , coding region , microbiology and biotechnology , genetics , escherichia coli , bacteria , membrane , receptor
We cloned and characterized the oprQ gene coding for outer membrane protein OprE3 of Pseudomonas aeruginosa PAO1. The oprQ gene was composed of 1,275 base pairs including a sequence encoding for the signal sequence and a mature protein with a Mr of 44,602. Computer‐aided alignment and hydropathy analyses of the predicted amino acid sequences suggested that OprE3 is a transmembrane protein homologous to outer membrane proteins of P. aeruginosa such as OprD2 (OprD) porin and OprE1 (OprE) porin. Susceptibility to several antibiotics of the strains lacking or overproducing OprE3 was indistinguishable from that of the wild‐type strain, suggesting that OprE3 is unlikely involved in the diffusion of carbapenems and other β‐lactam antibiotics.