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Genotypic Characterization of Human and Environmental Isolates of Salmonella choleraesuis Subspecies choleraesuis Serovar Infantis by Pulsed‐Field Gel Electrophoresis
Author(s) -
Murakami Koichi,
Horikawa Kazumi,
Otsuki Koichi
Publication year - 1999
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1999.tb02406.x
Subject(s) - biology , serotype , pulsed field gel electrophoresis , subspecies , genotype , salmonella , microbiology and biotechnology , outbreak , genetic diversity , strain (injury) , gel electrophoresis , genetic variability , virology , veterinary medicine , genetics , bacteria , gene , population , zoology , medicine , demography , anatomy , sociology
To determine the extent of genetic diversity of Salmonella choleraesuis subspecies choleraesuis serovar Infantis and whether environmental isolates were similar or identical to human isolates, a total of 110 isolates from humans, broiler samples, egg production facilities, riverwater, sewage, and chicken meat were analyzed epidemiologically by pulsed‐field gel electrophoresis. While the isolates showed 35 distinct pulsed‐field profiles, none had the genotype of the human isolates. One pulsed‐field profile was shared by 43 (39%) of the 110 isolates. These results indicate that relatively fewer clonal lines of S . serovar Infantis had spread widely while multiple clonal lines, including the strain involved in the outbreak, exist in Western Japan.