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Effective Inhibition of Candida albicans Growth by the Combination of Murine Peritoneal Neutrophils and Activated Macrophages
Author(s) -
Tansho Shigeru,
Abe Shigeru,
Tansho Tohru,
Yamaguchi Hideyo
Publication year - 1999
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1999.tb02398.x
Subject(s) - candida albicans , microbiology and biotechnology , spleen , lipopolysaccharide , biology , macrophage , population , immunology , in vitro , medicine , biochemistry , environmental health
The effect of leukocytes on the anti‐ Candida activity of neutrophils was examined. Murine neutrophils which were purified from casein‐induced peritoneal cells inhibited the mycelial growth of Candida albicans . This anti‐ Candida activity of neutrophils was augmented by the addition of spleen cells prepared from mice pretreated with bacterial lipopolysaccharide 3 hr before, but not from non‐treated mice. The population in the spleen cells, which enhanced the anti‐ Candida activity of neutrophils, was plastic‐plate adherent, nylon‐fiber columns adherent and anti‐Mac‐1 antigen‐positive. These immunological profiles suggested that the enhancing cells are classified to splenic macrophages. Peritoneal‐exudated macrophages from mice treated with lipopolysaccharide also augmented the anti‐ Candida activity of neutrophils. These results suggest that the anti‐ Candida activity of neutrophils may be upregulated by activated macrophages.