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Genotypic and Phenotypic Analysis of Bromovirus Adaptive Mutants Derived from a Single Plant
Author(s) -
Fujita Yasunari,
Mise Kazuyuki,
Furusawa Iwao
Publication year - 1999
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1999.tb02391.x
Subject(s) - biology , viral quasispecies , mutant , population , virology , brome mosaic virus , genotype , plant virus , gene , phenotype , mutation , genetics , cucumber mosaic virus , rna , virus , rna dependent rna polymerase , demography , sociology , hepatitis c virus
Eight adaptive mutant clones have been made from the total RNA extracted from uninoculated upper leaves of a single cowpea plant exhibiting systemic infection after inoculation with a hybrid cowpea chlorotic mottle bromovirus (CCMV) with the 3a movement protein gene of CCMV replaced by that of cowpea‐nonadapted brome mosaic bromovirus (BMV). Sequence and mutational analyses of these clones showed genotypic and phenotypic diversity of the cloned virus population, but all examined clones had the adaptive mutation, A to C at position 776 within the BMV 3a gene, required for the systemic infection of cowpea. The data support the quasispecies model for RNA virus population, and suggest that the maintenance of the adaptive mutation may be due to powerful selection pressure in an infection process.