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Difference in Response of NK Cell Activity in Newborns and Adults to IL‐2, IL‐12 and IL‐15
Author(s) -
Hoshina Takayuki,
Kida Kazunori,
Ito Masahiro
Publication year - 1999
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1999.tb02387.x
Subject(s) - peripheral blood mononuclear cell , k562 cells , biology , immunology , cord blood , interleukin 15 , interleukin , interleukin 12 , interleukin 2 , cytokine , cytotoxic t cell , in vitro , leukemia , biochemistry
The killing activity of cord blood mononuclear cells (cMNC) against cytomegalovirus (CMV)‐uninfected and ‐infected fibroblasts was comparable to that of adult peripheral blood mononuclear cells (aPBMC). The killing activity of cMNC against K562 cells was significantly lower compared with that of aPBMC. Treatment of cMNC and aPBMC with interleukin‐2 (IL‐2), IL‐12 or IL‐15 significantly enhanced killing activity against K562 cells and CMV‐uninfected and ‐infected cells. By comparison of cMNC with aPBMC, killing activity against the K562 cells of cMNC was augmented to the level of aPBMC when cultured with IL‐2, IL‐12 or IL‐15. The killing activity of cMNC against CMV‐uninfected and ‐infected fibroblasts did not increase to the level of adult PBMC by treatment with IL‐2, IL‐12 or IL‐15. These data suggest that cord blood contains a functionally different NK cell subpopulation than that among adult NK cells.