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Functional Characteristics of Human Peripheral Blood α/βTCR+, CD4‐ and CD8‐Double‐Negative (DN) T Cells
Author(s) -
Niehues Tim,
Eichelbauer Dirk,
Schneider E. Marion
Publication year - 1999
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1999.tb02386.x
Subject(s) - biology , cd8 , in vivo , in vitro , microbiology and biotechnology , double negative , cytotoxic t cell , cd3 , t cell , immunology , immune system , biochemistry
Abstract The function of human peripheral blood α/βTCR positive, CD4‐ and CD8‐ double‐negative T lymphocytes (DN cells) in vivo is not completely understood. The response of immunomagnetically isolated DN cells to PHA and anti‐CD3 was compared to the response of single‐positive (SP) CD4 and CD8 subsets. Proliferation of DN cells in response to PHA was largely independent of APC. This suggests activation requirements for DN cells that are different from SP cells. Upon activation, HLA‐DR was found to be upregulated early on DN cells, and IL‐4 and IL‐10 were detected in the supernatants of DN cells. These observations in vitro could correspond with an immunoregulatory role of human DN cells in vivo .