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Anti‐HIV‐1 Activity of an lonically Modified Porous Polypropylene Membrane Determined by Filtration of a Viral Suspension
Author(s) -
Owada Takashi,
Miyashita Yuko,
Motomura Tadahiro,
Onishi Makoto,
Yamashita Shuzo,
Yamamoto Naoki
Publication year - 1999
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1999.tb02385.x
Subject(s) - titer , infectivity , virology , peripheral blood mononuclear cell , virus , hiv antigens , antigen , antibody , biology , immunology , viral disease , in vitro , biochemistry
We describe here a unique anti‐HIV‐1 membrane, derived from a chemically modified porous polypropylene (PP) membrane, which lowers viral infectivity upon the filtration of HIV‐1 suspension. A cationic polymer, polyethyleneimine (PEI) was graft‐polymerized onto the PP filter membrane (PP‐PEI), and infectious HIV‐1 HTLV‐IIIB derived from MOLT‐4/HIV‐1 HTLV‐IIIB cells (HIV‐1 HTLV‐IIIB(MOLT‐4) ) was applied. When a viral suspension of high titer (10 3.93 TCID 50 ml 1 ) was filtered, efficient reduction (>99%) of gag p24 antigen levels and infectious titer resulted. In a viral suspension of medium titer (10 2.37 TCID 50 ml 1 ), a significant decrease in the p24 antigen did not occur, although the titer was markedly reduced (>95%). Electron microscopic observation suggested that PEI induced viral aggregations under high titer conditions, and under medium titer conditions, PEI deprived HIV‐1 HTLV‐IIIB of its infectivity alone to avoid virus adsorption. In contrast, HIV‐1 propagated in human peripheral blood mononuclear cells (PBMC) such as HIV‐1 HTLV‐IIIB(PBMC) was more efficiently trapped by PP‐PEI at lower titers as compared with HIV‐1 HTLV‐IIIB(MOLT‐4) from MOLT‐4/HIV‐1 HTLV‐IIIB cells. These data suggest host cell modification in the interactions between PP‐PEI and HIV‐1 strains. Since HIV‐1 HTLV‐IIIB(MOLT‐4) and HIV‐1 HTLV‐IIIB(PBMC) were almost electrically neutral and negative, respectively, we concluded that the divergent effect of PEI on each HIV‐1 HTLV‐IIIB resulted from their different electrical characteristics.