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Protective Immunity Induced by Vaccination with SAG1 Gene‐Transfected Cells against Toxoplasma gondii ‐Infect ion in Mice
Author(s) -
Aosai Fumie,
Mun Hyeseong,
Norose Kazumi,
Chen Mei,
Hata Hidekazu,
Kobayashi Masashi,
Kiuchi Masahiro,
Stauss Hans J.,
Yano Akihiko
Publication year - 1999
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1999.tb02378.x
Subject(s) - toxoplasma gondii , biology , virology , cytotoxic t cell , immunization , vaccination , ctl* , antigen , immunity , cd8 , transfection , immunology , virulence , immune system , antibody , gene , in vitro , genetics
To develop a vaccine by augmenting the protective cellular immunity against Toxoplasma gondii (T. gondii )‐infection, T. gondii SAG1 gene‐transfectants were established by using RMA.S (H‐2 b ), a murine transporter associated with the antigen processing (TAP) molecule‐deficient lymphoma line, as a host antigen‐presenting cell (APC). Immunization of C57BL/6 mice with the SAG1‐transfected RMA.S induced CD8 + cytotoxic T lymphocytes (CTL) specific for not only SAG1‐transfected RMA.S but also T. gondii ‐infected RMA.S, and elicited protective responses to infection with a virulent T. gondii strain, RH.