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Expression of Lymphotoxin Gene Inserted into Theiler's Murine Encephalomyelitis Virus
Author(s) -
Obuchi Masatsugu,
Odagiri Takato,
Lizuka Hideaki,
Ohara Yoshiro
Publication year - 1999
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1999.tb02377.x
Subject(s) - lymphotoxin , biology , virology , virus , recombinant virus , recombinant dna , gene , microbiology and biotechnology , immunology , immune system , genetics
Theiler's murine encephalomyelitis virus (TMEV) belongs to the Picornaviridae genus. DA subgroup strains of this virus induce early, non‐fatal polioencephalomyelitis followed by demyelination in the spinal cord, with virus persistence. We investigated the use of DA strain as a vector for the introduction of a foreign gene into the central nervous system. Human lymphotoxin (LT) gene was inserted in the L region, the most upstream part of the polyprotein coding region of DA genome. Expression of LT was demonstrated by an immunoblot and an enzyme‐linked immunosorbent assay on BHK‐21 cells that were infected with the recombinant virus. In addition, the expressed LT showed cytotoxicity against L‐929 cells.