Premium
Growth Conditions of and Emetic Toxin Production by Bacillus cereus in a Defined Medium with Amino Acids
Author(s) -
Agata Norio,
Ohta Michio,
Mori Masashi,
Shibayama Keigo
Publication year - 1999
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1999.tb02367.x
Subject(s) - bacillus cereus , valine , toxin , isoleucine , amino acid , leucine , biology , biochemistry , threonine , microbiology and biotechnology , cereus , bacteria , serine , enzyme , genetics
The growth and emetic toxin (cereulide) production of Bacillus cereus strains in defined culture media were studied. We found that a fully synthetic medium (CADM) allowed the production of emetic toxin and the addition of glucose enhanced it. By subtracting each amino acid from CADM, we found that only three amino acids, valine, leucine and threonine, were essential for growth and toxin production by B. cereus . The addition of high levels (50 mM) of leucine, isoleucine and glutamic acid decreased the toxin production. Other amino acids had no effect at this concentration.