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New Monoclonal Antibodies against a Recombinant Second Envelope Protein of Hepatitis C Virus
Author(s) -
Inudoh Michiharu,
Kato Nobuyuki,
Tanaka Yuetsu
Publication year - 1998
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1998.tb02364.x
Subject(s) - monoclonal antibody , biology , virology , microbiology and biotechnology , chinese hamster ovary cell , antibody , virus , recombinant dna , hepatitis e virus , hepatitis c virus , viral envelope , parvovirus , cell culture , immunology , biochemistry , genetics , gene , genotype
To study the immunological features of the hepatitis C virus (HCV) envelope protein (E2 protein), new specific monoclonal antibodies (mAbs) were generated. WKA/H rats were immunized with syngeneic cells infected with a vaccinia virus expressing the E2 protein and with soluble E2 protein obtained from Chinese hamster ovary cells with a plasmid‐based expression system. By screening hybridoma cells obtained from spleen cells of the immunized rats, three specific mAbs were obtained. One mAb was reactive to a peptide corresponding to the hypervariable region 1 (HVR1) in E2 protein, while the others reacted to regions outside HVR1. The significance of these antibodies for the diagnosis of HCV infection as well as for analysis of the structure of the HCV E2 protein will be discussed.