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Anti‐ Candida Activity of Cal protect in in Combination with Neutrophils or Lactoferrin
Author(s) -
Okutomi Takafumi,
Tanaka Takashi,
Yui Satoru,
Mikami Masaaki,
Yamazaki Masatoshi,
Abe Shigeru,
Yamaguchi Hideyo
Publication year - 1998
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1998.tb02353.x
Subject(s) - calprotectin , lactoferrin , candida albicans , microbiology and biotechnology , corpus albicans , biology , effector , immunology , biochemistry , medicine , inflammatory bowel disease , disease
The effect of an anti‐microbial protein, calprotectin, in combination with neutrophils on the growth of Candida albicans was investigated. The growth inhibition of C. albicans by murine neutrophils was augmented by the addition of a low concentration of calprotectin prepared from rat peritoneal exudate cells. The concentrations of calprotectin causing 50% inhibition of growth of C. albicans in the absence or presence of neutrophils at an effector‐to‐target (E/T) ratio of 30 and 60 were estimated to be 0.45, 0.34 and 0.28 U/ml, respectively. The anti‐ Candida activity of calprotectin was completely inhibited by 2 μM of zinc ion, while it only partially lowered the activity of the combination of calprotectin and neutrophils. Lactoferrin, which is an anti‐microbial protein released from neutrophils, strongly inhibited the growth of C. albicans in combination with calprotectin. These results suggest that calprotectin and lactoferrin released from neutrophils may cooperate to inhibit the growth of C. albicans at a local lesion of the infection where there is an accumulation of neutrophils.