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Analysis of Genetic Relationships and Antimicrobial Susceptibility of Verotoxin‐Producing Escherichia coli Strains Isolated in Nagasaki Prefecture, Japan in 1996
Author(s) -
Izumikawa Koichi,
Hirakata Yoichi,
Yamaguchi Toshiyuki,
Yoshida Ryoji,
Nakano Michiko,
Matsuda Junichi,
Mochida Chikako,
Maesaki Shigefumi,
Tomono Kazunori,
Yamada Yasuaki,
Tashiro Takayoshi,
Kohno Shigeru,
Kamihira Shimeru
Publication year - 1998
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1998.tb02339.x
Subject(s) - pulsed field gel electrophoresis , biology , microbiology and biotechnology , amikacin , outbreak , ampicillin , escherichia coli , trimethoprim , vtec , fosfomycin , sulfamethoxazole , ciprofloxacin , antimicrobial , antibiotics , virology , genotype , genetics , gene
A total of 19 Escherichia coli O157 isolates were obtained in Nagasaki Prefecture, in the southwestern part of Japan, between 1990 and 1996. Pulsed‐field gel electrophoresis (PFGE) and computer‐assisted analysis were applied to determine genetic relationships among these strains. Fragment patterns of the isolates in Nagasaki, as determined by PFGE, were compared with those of isolates in other areas where large outbreaks and sporadic cases of E. coli O157 infection occurred. Similarity values of all the strains isolated in Nagasaki Prefecture were over 0.65 except for E. coli O26. Some strains were identical to the strains isolated from the areas where large outbreaks occurred. All strains were susceptible to ampicillin, fosfomycin, minocycline, amikacin, ofloxacin and sulfamethoxazole‐trimethoprim.