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Regulation of GM‐CSF‐Induced Dendritic Cell Development by TGF‐β1 and Co‐Developing Macrophages
Author(s) -
Yamaguchi Yasunori
Publication year - 1998
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1998.tb02333.x
Subject(s) - cd86 , microbiology and biotechnology , biology , progenitor cell , dendritic cell , transforming growth factor , mhc class ii , immunology , major histocompatibility complex , t cell , antigen , stem cell , immune system
Using a culture system of bone marrow progenitor cells with GM‐CSF and TGF‐β1, a study was performed to analyze the effect of TGF‐β1 on the development of dendritic cells (DC) and to elucidate the regulatory role of macrophages co‐developing with dendritic cells. The results demonstrate that DC generated in the presence of TGF‐β1 were immature with respect to the expression of CD86, nonspecific esterase activity and cell shape. Such inhibitory effects of TGF‐β1 were dependent on FcR + macrophages, which were depleted by panning. TGF‐β1 did not appear to inhibit the commitment of progenitor cells to the DC lineage. In addition, TGF‐β1 also acted directly on the intermediate stage of DC to prevent their over‐maturation, which results in a preferential decrease in MHC class II, but not in CD86, in the presence of TNF‐α. FcR + suppressive macrophages were also shown to facilitate DC maturation when stimulated via FcR‐mediated signals even in the presence of TGF‐β1. These results indicate that TGF‐β1 indirectly and directly regulate the development of DC and that co‐developing macrophages have a regulatory role in DC maturation.