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Rapid Analysis of Allelic Variants of the Sheep PrP Gene by Oligonucleotide Probes
Author(s) -
Ishiguro Naotaka,
Shinagawa Morikazu,
Onoe Sadao,
Yamanouchi Kazunori,
Saito Toshiro
Publication year - 1998
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1998.tb02327.x
Subject(s) - biology , allele , scrapie , gene , polymerase chain reaction , flock , microbiology and biotechnology , oligonucleotide , oligomer restriction , southern blot , genetics , dna , prion protein , medicine , paleontology , disease , pathology
A rapid method to determine the allelic variants of the sheep PrP gene was developed. DNA samples from 128 Suffolk sheep (39 rams and 89 ewes) were screened by using polymerase chain reactions and dot‐blot hybridization with 32 P‐labeled nine allele‐specific oligonucleotide probes corresponding to the polymorphic PrP codons 112, 136, 154 and 171. Three allelic variants of the PrP gene, PrP MARQ , PrP TARQ and PrP MARR , were found in the flocks. Among those variants, nearly half of the ewes had alleles of the 171‐Arg variant that is closely associated with resistance to natural scrapie. Assessments of allelic mutations of the PrP gene may help to select the scrapie‐resistant progenitors in the flocks.