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Combined Use of Bacteriophage Typing and Pulsed‐Field Gel Electrophoresis in the Epidemiological Analysis of Japanese Isolates of Enterohemorrhagic Escherichia coli O157:H7
Author(s) -
Izumiya Hidemasa,
Masuda Takashi,
Ahmed Rafiq,
Khakhria Rasik,
Wada Akihito,
Terajima Jun,
Itoh Kenichiro,
Johnson Wendy M.,
Konuma Hirotaka,
Shinagawa Kunihiro,
Tamura Kazumichi,
Watanabe Haruo
Publication year - 1998
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1998.tb02318.x
Subject(s) - biology , pulsed field gel electrophoresis , escherichia coli , bacteriophage , microbiology and biotechnology , typing , gel electrophoresis , phage typing , molecular epidemiology , virology , genetics , genotype , gene
A total of 236 enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates in Japan were investigated by bacteriophage typing, and the results were compared with those of pulsed‐field gel electrophoresis (PFGE). Seven phage types (PTs) were observed in 71 isolates which were derived from 22 outbreaks. All of the isolates from ten outbreaks in the Kinki region (midwestern part of Japan) in July‐August 1996 were grouped into the same PFGE type (IIa) and PT 32, while among total isolates, there were such varieties as PFGE type IIa containing five phage types and PT32 containing two PFGE types. These results suggest that the ten outbreaks should be considered to be a single outbreak, and show that the combined use of bacteriophage typing and PFGE enhances reliability in epidemiological surveys.