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Differentiation of Chlamydia Species by Combined Use of Polymerase Chain Reaction and Restriction Endonuclease Analysis
Author(s) -
Yoshida Hiroshi,
Kishi Yuichiro,
Shiga Sadashi,
Hagiwara Toshikatsu
Publication year - 1998
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1998.tb02303.x
Subject(s) - biology , restriction enzyme , chlamydia psittaci , primer (cosmetics) , polymerase chain reaction , chlamydia , endonuclease , chlamydiaceae , chlamydia trachomatis , dna , microbiology and biotechnology , polymerase , chlamydiales , gene , genetics , virology , chemistry , organic chemistry
To differentiate Chlamydia spp., a primer pair designed to generate a genus‐specific region of the major outer membrane protein (MOMP) gene was used in a PCR to amplify a single DNA fragment of 245‐259 bp. In the PCR, the expected single DNA fragment was amplified from strains of Chlamydia trachomatis, C. psittaci, C. pneumoniae and C. pecorum , respectively. By restriction endonuclease analysis with Alu I and Pvu II, the amplified products exhibited four distinct patterns, corresponding to the four species. It is, therefore, concluded that one‐step PCR followed by restriction endonuclease analysis as described in this study could be a valuable method for the detection and differentiation of Chlamydia species.