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Evidence for a Dual Strategy in the Expression of Cauliflower Mosaic Virus Open Reading Frames I and IV
Author(s) -
Kobayashi Kappei,
Tsuge Seiji,
Nakayashiki Hitoshi,
Mise Kazuyuki,
Furusawa Iwao
Publication year - 1998
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1998.tb02291.x
Subject(s) - cauliflower mosaic virus , subgenomic mrna , biology , orfs , open reading frame , gene , microbiology and biotechnology , rna , gene expression , genetics , virology , transgene , genetically modified crops , peptide sequence
Studies have indicated that cauliflower mosaic virus (CaMV) gene expression is mediated by the translation of polycistronic 35S pregenomic RNA, but the involvement of some minor subgenomic RNA species is also suspected. We examined the involvement of the 35S promoter in the expression of CaMV open reading frames (ORFs) I and IV using both 35S RNA‐driven and promoter‐less ORF I‐ and ORF IV‐β‐glucuronidase (GUS) fusion constructs. In addition to the 35S promoter‐dependent expression of both ORF I‐ and IV‐GUS fusions, we detected the 35S promoter‐independent expression of both fusion genes via subgenomic mRNAs, which were detected by Northern blotting in the protoplasts transfected with the 35S promoter‐driven constructs as well as in those transfected with the promoter‐less constructs. These results suggest the involvement of subgenomic RNAs in the expression of CaMV ORFs I and IV, and the operation of a dual strategy in the expression of two viral genes.