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Human Immunodeficiency Virus Type 1 (HIV‐1) Infection and Transcytosis Activity of a HIV‐1 Susceptible Clone from HeLa Cell
Author(s) -
Morizono Kouki,
Harada Shinji
Publication year - 1998
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1998.tb02288.x
Subject(s) - biology , virology , transcytosis , hela , clone (java method) , human immunodeficiency virus (hiv) , virus , lentivirus , cell , immunology , viral disease , gene , genetics , endocytosis
In order to clarify the transmission process of human immunodeficiency virus type 1 (HIV‐1) through the epithelial cell barrier, HeLa cells susceptible and non‐susceptible to HIV‐1 were cloned and designated as P6 HeLa and N7 HeLa cells, respectively. P6 HeLa cells could be infected with the LAI strain of HIV‐1 and mediated HIV‐1 transcytosis. In contrast, N7 HeLa cells exhibited neither HIV‐1 infection nor transcytosis. CD4 and galactosylceramide as the receptors for HIV‐1 were not detected on P6 HeLa cells, although an anti‐CD4 monoclonal antibody (mAb) blocked HIV‐1 infection. Since HIV‐1‐infected P6 HeLa cells exhibited no fusion and survived, we speculated that the P6 HeLa cells expressed molecules other than CD4 which facilitated HIV‐1 infection. Two mAbs (A‐14 ITK and C57 a9‐9) which inhibited the HIV‐1 infection of P6 HeLa cells were generated. Each mAb recognized distinct molecule(s) as shown by Western blotting. Transcytosis by the P6 HeLa cells was inhibited by C57 a9‐9 but not by A‐14 ITK or anti‐CD4 mAb. Both infection and transcytosis may be responsible for HIV‐1 transmission through epithelial cells in a complex manner. Although infection and transcytosis occurred via different mechanisms, the molecule(s) recognized by C57 a9‐9 mAb may be associated with both processes.