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Multiplication of Legionella pneumophila in HeLa Cells in the Presence of Cytoskeleton and Metabolic Inhibitors
Author(s) -
Goldoni Paola,
Cattani Lorena,
Carrara Stefania,
Pastoris Maddalena Castellani,
Sinibaldi Laura,
Orsi Nicola
Publication year - 1998
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1998.tb02283.x
Subject(s) - intracellular , legionella pneumophila , biology , cytoskeleton , hela , cytochalasin b , cytochalasin , microbiology and biotechnology , cytochalasin d , biochemistry , cell , bacteria , genetics
A study has been carried out on the action of cytoskeleton and metabolic inhibitors on intracellular multiplication in HeLa cells of a virulent strain of Legionella pneumophila serogroup 6. The effects of the substances were separately tested on both penetration and intracellular multiplication of L. pneumophila . Only cytochalasin A and 2‐deoxy‐ d ‐glucose (2dG) affected bacterial internalisation, whereas intracellular multiplication was inhibited by cytochalasins A, B, C, D and J (D being the most active) and by 2dG with a dose‐response effect. The action of 2dG was counteracted by 50 mM glucose. Experiments carried out with cytochalasin D and a rhodamine‐phalloidin conjugate showed the involvement of cytoskeletal elements in intracellular multiplication of Legionella ; compounds acting on microtubules had no effect.