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Response of Blood Platelets to Proteus mirabilis Lipopolysaccharide
Author(s) -
Wachowicz Barbara,
Saluk Joanna,
Kaca Wiesław
Publication year - 1998
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1998.tb01968.x
Subject(s) - tbars , superoxide , platelet , proteus mirabilis , lipopolysaccharide , thromboxane a2 , thiobarbituric acid , reactive oxygen species , thromboxane , biology , biochemistry , chemistry , endocrinology , immunology , lipid peroxidation , enzyme , escherichia coli , gene
The effects of the lipopolysaccharide (LPS) of Proteus mirabilis on the production of thiobarbituric acid reactive substances (TBARS) and the generation of superoxide radicals (O 2 − ) by pig blood platelets were studied in vitro. The effect of LPS on TBARS formation in platelets was dependent on the concentration of endotoxin. LPS at concentrations above 0.1 μg/10 8 platelets caused the production of TBARS concomitant with the generation of superoxide radicals. The responses of platelets to LPS suggest that endotoxin, like thrombin (a strong platelets agonist), stimulates an enzymatic cascade of platelet arachidonate via cyclooxygenase and produces thromboxane A 2 (TXA 2 ) concomitant with malonyldialdehyde (MDA).