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Shotgun Cloning and Characterization of the Thymidylate Synthase‐Encoding Gene from Mycobacterium bovis BCG
Author(s) -
Matsumoto Sohkichi,
Yukitake Hideharu,
Ohara Naoya,
Dairi Tohru,
Kanbara Hiroji,
Yamada Takeshi
Publication year - 1998
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1998.tb01964.x
Subject(s) - biology , thymidylate synthase , mycobacterium bovis , cloning (programming) , gene , shotgun sequencing , shotgun , genetics , microbiology and biotechnology , mycobacterium , computational biology , mycobacterium tuberculosis , dna sequencing , bacteria , tuberculosis , cancer , medicine , pathology , computer science , programming language , fluorouracil
The shotgun cloning of a Mycobacterium bovis BCG (BCG) genome into pBluescript SK (+) successfully yielded a 0.9 kbp fragment, confirming the ability of Escherichia coli thyA mutant MH2702 to grow in a thymine‐depleted medium. This DNA fragment contained a gene homologous to the thymidylate synthase (TS)‐encoding genes (thyA) of other organisms. An inverted repeat sequence and open reading frame (ORF) were observed at the upstream region of the thyA. A computer analysis revealed that the protein encoded by this ORF possessed a structure unique for a DNA binding protein.