Premium
Structural and Immunochemical Studies of Two Cross‐Reactive Proteus mirabilis O‐Antigens, O6 and O23, Containing β1→3‐Linked 2‐Acetamido‐2‐Deoxy‐ d ‐Glucopyranose Residues
Author(s) -
Cedzyñski Maciej,
Świerzko Anna St,
Zi´łkowski Andrzej,
Różalski Antoni,
Paramonov Nikolay A.,
Vinogradov Evgeny V.,
Knirel Yuriy A.,
Kaca Wiesław
Publication year - 1998
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1998.tb01963.x
Subject(s) - proteus mirabilis , antiserum , biology , epitope , lipopolysaccharide , polysaccharide , antigen , cross reactivity , strain (injury) , proteus , microbiology and biotechnology , biochemistry , flagellum , stereochemistry , chemistry , escherichia coli , cross reactions , anatomy , genetics , immunology , gene
A marked serological cross‐reactivity was observed by ELISA and a precipitation test between anti‐ Proteus mirabilis O23 serum and the lipopolysaccharide as well as the O‐specific polysaccharide from the Proteus mirabilis strain belonging to serogroup O6. The structures of the O‐specific polysaccharides were elucidated using chemical and NMR spectroscopic analyses, and the only common component, 2‐acetamido‐2‐deoxy‐β‐ d ‐glucopyranose (β‐ d ‐GlcNAc), was revealed, which was suggested to be responsible for the cross‐reactivity observed. Both anti‐O23 and anti‐O6 sera were shown to react with 1, 3‐linked β‐ d ‐GlcNAc‐containing O‐antigen from Salmonella enterica ssp. arizonae O59 also. The lack of reactivity of Smith‐degraded P. mirabilis O6 O‐specific polysaccharide with homologous antiserum indicated the crucial role of α‐ d ‐glucuronic acid in specific antibody binding.