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Mechanisms of Allografted Tumor Rejection: The Roles of T Cells in Allograft Rejection Mediated by a Type of Bone Marrow‐Derived Macrophage
Author(s) -
UshioUmeda Yumiko,
Yoshida Ryotaro
Publication year - 1997
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1997.tb01958.x
Subject(s) - bone marrow , cd8 , lymph node , adoptive cell transfer , immunology , biology , cytotoxic t cell , progenitor cell , t cell , immune system , cancer research , stem cell , microbiology and biotechnology , in vitro , biochemistry
Allografted tumor rejection does not occur in the absence of T cells, but the main effector cells responsible for the rejection are allograft‐induced macrophages (AIM). We examined the roles of T cells in the AIM‐mediated rejection of Meth A (H‐2) tumor cells from C57BL/6 (H‐2 b ) mice. Irradiation of C57BL/6 mice abrogated both the induction of AIM and the allograft rejection. Reconstitution of the irradiated mice with F1 (C57BL/6 X C3H/He: H‐2 b/k ) bone marrow cells led to the appearance of H‐2 b/k haplo‐type of AIM exclusively in the rejection site and to allograft rejection, indicating that radiosensitive cells prerequisite for both the induction of AIM and allograft rejection were bone marrow‐derived cells, and that the progenitors of AIM existed in the bone marrow cells to be activated into AIM in the rejection site. To understand the role of T cells in the induction of AIM, we used adult‐thymectomized, X‐irradiated C57BL/6 mice reconstituted with F1 bone marrow (ATXBM). The ATXBM mice could neither induce AIM nor reject allogeneic Meth A cells, whereas adoptive transfer of F1 lymph node T cells to the ATXBM mice restored not only the induction of AIM but also rejection of the allograft. Among the lymph node T cells, CD4 + , but not CD8 + , cells were found to be essential for the activation of AIM progenitors to AIM; and CD8 + T cells were further required for rejection, at least in part, to enhance the number of AIM in the rejection site.