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Evaluation of Enzyme‐Labeled Oligonucleotide Probes to Identify Enterohaemorrhagic Escherichia coli
Author(s) -
Yoh Myonsun,
Takagi Kazuhiro,
Eda Junji,
Ohtomo Masato,
Takarada Yutaka,
Shibata Shuji,
Honda Takeshi
Publication year - 1997
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1997.tb01944.x
Subject(s) - shigella dysenteriae , escherichia coli , biology , shigella , microbiology and biotechnology , shiga toxin , latex fixation test , vero cell , molecular probe , enterobacteriaceae , hybridization probe , virology , gene , virus , biochemistry , genetics , antibody
Alkaline phosphatase‐conjugated oligonucleotide probes were developed to detect the gene coding for Vero toxin 1 (VT1) and Vero toxin 2 (VT2). Using these probes, 3 hr was enough to detect VT genes when suspicious colonies of enterohaemorrhagic Escherichia coli (EHEC) were obtained on an agar plate. The results of a hybridization test with 144 isolates of EHEC O157 and one isolate of Shigella dysenteriae Type 1 agreed exactly with the immunological detection, reversed passive latex agglutination (RPLA) test, of VTs in their culture supernatants. The sensitivity levels of these probes for the detection of VT genes were 100%. The specificity of these probes were also tested with a total of 1,002 strains of Escherichia coli other than EHEC and 8 strains of Shigella sp. other than Shigella dysenteriae Type 1; the results showed 100% specificity.