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Hyaluronidase Activity in Human Pus from Which Streptococcus intermedius Was Isolated
Author(s) -
Takao Ayuko,
Nagashima Hiroyuki,
Usui Hiroyuki,
Sasaki Fumihiko,
Maeda Nobuko,
Ishibashi Katsunori,
Fujita Hiroshi
Publication year - 1997
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1997.tb01929.x
Subject(s) - streptococcus intermedius , biology , hyaluronidase , antiserum , zymography , streptococcaceae , microbiology and biotechnology , in vivo , in vitro , streptococcus , enzyme , bacteria , biochemistry , immunology , antigen , genetics , antibiotics
Hyaluronidase (HAase) activity was detected in both a human pus sample and the culture supernatant of the only bacterial isolate from the pus, Streptococcus intermedius , using a zymographic technique. The optimum pH range for HAase activity was similar for both samples. Although the bands showing the strongest HAase activity of these samples differed from each other with respect to molecular size, both samples were equally inhibited by an antiserum raised against HAase of S. intermedius. These results suggest that S. intermedius may produce HAase in vivo as well as in vitro , and that this enzyme and/or its fragments may play an important role in host tissue degradation.