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Amplification of rfbE and fliC Genes by Polymerase Chain Reaction for Identification and Detection of Salmonella Serovar Enteritidis, Dublin and Gallinarum‐Pullorum
Author(s) -
Itoh Yoshihiro,
Hirose Kenji,
Miyake Masaki,
Khan Abdul Q.,
Hashimoto Yasuhiro,
Ezaki Takayuki
Publication year - 1997
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1997.tb01928.x
Subject(s) - biology , polymerase chain reaction , serotype , salmonella enteritidis , microbiology and biotechnology , salmonella , gene , identification (biology) , virology , genetics , bacteria , botany
Polymerase chain reaction (PCR) primers for O9 antigen ( rfbE ) and phase 1 flagellin antigen ( fliC ) were designed for the rapid identification and detection of Salmonella serovar Enteritidis and Dublin. The rfbE primer pairs selectively amplified the rfbE region of group O9 Salmonella serovars. The fliC primer pairs amplified the DNAs of g,m and g,p‐type flagellar antigen; Salmonella serovar Enteritidis, Dublin, and Essen. However, DNA from flagellar‐negative Salmonella serovar Gallinarum‐Pullorum was also amplified. The sensitivity of PCR primer pairs was 10 CFU/assay by boiled DNA preparation and 10 2 CFU/assay by proteinase K‐treated DNA preparation.