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Triazine Dyes Inhibit HIV‐1 Entry by Binding to Envelope Glycoproteins
Author(s) -
Hattori Toshio,
Zhang Xiaoyan,
Weiss Carol,
Xu Youg,
Kubo Toru,
Sato Yoshimi,
Nishikawa Shigemichi,
Sakaida Hitoshi,
Uchiyama Takashi
Publication year - 1997
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1997.tb01916.x
Subject(s) - glycoprotein , triazine , biology , albumin , agarose , biochemistry , bovine serum albumin , viral envelope , microbiology and biotechnology , chemistry , polymer chemistry
We have attempted to purify envelope (Env) glycoproteins of human immunodeficiency virus (HIV) from the culture supernatants of CHO‐Sec cells that secreted truncated 140‐kDa precursor and mature 120‐kDa Env glycoproteins. The concentrated culture supernatants were applied to a column coupled with cibacron blue 3GA (CB3GA) to separate albumin from the Env proteins because CB3GA, a triazine dye, has been known to have a high affinity to albumin. Unexpectedly, Env proteins as well as albumin bound to the column, and the bound Env proteins were eluted by increasing the ionic strength using KC1. Gp120 was eluted at 0.5–0.9 m of KC1, while a higher concentration (0.9–1.5 m ) was necessary for the elution of gp140. The agarose gel coupled with reactive red 120 (RR120), another triazine dye with similar characteristics, also retained both Env proteins, and the bound Env proteins could be eluted in a similar manner. In addition, these agents inhibited syncytium formation caused by HTLV‐III B and HTLV‐II MN . Inhibition was also seen when a virus‐free fusion assay between Env protein expressed in CHO cells and fluorescent labeled SupT1 cells were used. These findings indicate that triazine dyes bind to the functional regions of Env proteins of HIV‐1 that play important role(s) for HIV infection.