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Combined Use of Ribotyping, PFGE Typing and IS 431 Typing in the Discrimination of Nosocomial Strains of Methicillin‐Resistant Staphylococcus aureus
Author(s) -
Yoshida Tatsumi,
Kondo Noriko,
Hanifah Yasmin Abu,
Hiramatsu Keiichi
Publication year - 1997
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1997.tb01912.x
Subject(s) - ribotyping , typing , genotyping , pulsed field gel electrophoresis , microbiology and biotechnology , biology , phage typing , staphylococcus aureus , genotype , restriction fragment length polymorphism , restriction enzyme , genetics , bacteria , gene
We have previously reported the phenotypic characterization of methicillin‐resistant Staphylococcus aureus (MRSA) clinical strains isolated in Malaya University Hospital in the period 1987 to 1989 using antibiogram, coagulase typing, plasmid profiles, and phage typing. Here, we report the analysis of the same strains with three genotyping methods; ribotyping, pulsed‐field gel electrophoresis (PFGE) typing, and IS431 typing (a restriction enzyme fragment length polymorphism analysis using an IS 431 probe). Ribotyping could discriminate 46 clinical MRSA strains into 5 ribotypes, PFGE typing into 22 types, and IS 431 typing into 15 types. Since the differences of the three genotyping patterns from strain to strain were quite independent from one another, the combined use of the three genotyping methods could discriminate 46 strains into 39 genotypes. Thus, the powerful discriminatory ability of the combination was demonstrated.