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Genomic Diversity in the pfoA Region of Theta‐Toxin‐Deficient Strains of Clostridium perfringens
Author(s) -
BaThein William,
Inui Satoko,
Shimizu Tohru,
Swe Tint,
Banu Sayera,
Ohtani Kaori,
Oe Misari,
Sakurai Naomi,
Nakamura Shinichi,
Hayashi Hideo
Publication year - 1997
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1997.tb01903.x
Subject(s) - toxin , clostridium perfringens , biology , gene , microbiology and biotechnology , arginine , enterotoxin , genetics , bacteria , escherichia coli , amino acid
The genomic structure of the pfoA‐colA region in six theta‐toxin‐deficient strains of Clostridium perfringens was examined by Southern hybridization using the pfoR , pfoA , pbg , arcABDC and colA genes, encoding regulator for pfoA , theta‐toxin, beta‐galactosidase, arginine metabolism enzymes and kappa‐toxin, respectively, as gene probes. It is suggested that the productivity of theta‐toxin in these strains is diverse because of the multiple genetic backgrounds including single deletion of pfoA , large deletion of the pfoA‐colA region and the putative point mutations.