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Biovar Diversity Is Reflected by Variations of Genes Encoding Urease of Ureaplasma urealyticum
Author(s) -
Ruifu Yang,
Minli Zhang,
Zhaobiao Guo,
Xiaohui Wang
Publication year - 1997
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1997.tb01902.x
Subject(s) - ureaplasma urealyticum , biovar , biology , gene , microbiology and biotechnology , phylogenetic tree , genetics , serotype , ureaplasma , urease , enzyme , mycoplasma , biochemistry
Five oligonucleotide primers derived from the gene encoding urease of Ureaplasma urealyticum were designed to evaluate the relationship between the urease gene and biovar diversity of this organism. Five combinations of these primers were tested by PCR and the result revealed that there were variations in urease genes among different serovars of U. urealyticum. This result, in agreement with other PCRs based on other functionally unrelated (rRNA and MB antigen) genes, may reflect the phylogenetic relationship among organisms taxonomically classified as U. urealyticum.