Premium
cDNA Cloning and Characterization of Rat C5a Anaphylatoxin Receptor
Author(s) -
Akatsu Hiroyasu,
Miwa Takashi,
Sakurada Chikai,
Fukuoka Yoshihiro,
Ember Julia A.,
Yamamoto Takayuki,
Hugli Tony E.,
Okada Hidechika
Publication year - 1997
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1997.tb01894.x
Subject(s) - c5a receptor , complementary dna , biology , anaphylatoxin , antiserum , polyclonal antibodies , complement system , receptor , microbiology and biotechnology , cdna library , transfection , cloning (programming) , messenger rna , immunology , gene , antibody , genetics , computer science , programming language
Activation of the complement cascade plays an esssential role in the early stages of inflammation. C5a and its receptor are particularly active in anaphylaxis. To determine the pathological roles played by C5a and C5a receptor (C5aR) in rats, we cloned C5aR cDNA and analyzed distribution of its mRNA in various organs including lung from an LPS‐stimulated rat. Furthermore, we generated a polyclonal antiserum which specifically recognizes rat C5aR, as confirmed by its specific interaction with cells transfected with rat C5aR cDNA.