Premium
Prevalence of Genotypes of Orientia tsutsugamushi in Patients with Scrub Typhus in Miyazaki Prefecture
Author(s) -
Horinouchi Hisato,
Murai Koichi,
Okayama Akihiko,
Nagatomo Yasuhiro,
Tachibaobuyoshi,
Tsubouchi Hirohito
Publication year - 1997
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1997.tb01884.x
Subject(s) - orientia tsutsugamushi , scrub typhus , biology , genotype , polymerase chain reaction , strain (injury) , virology , rickettsiosis , restriction fragment length polymorphism , gene , genetics , rickettsia , anatomy , virus
Abstract The genotypes of Orientia tsutsugamushi in patients with scrub typhus in Miyazaki Prefecture were examined by polymerase chain reaction (PCR) and the restriction fragment length polymorphism method. Specific patterns for genotypes Irie, Hirano, Tazume and Yoshimura were detected in 26, 6, 5 and 2 of 39 DNA samples obtained from peripheral blood mononuclear cells, respectively. DNA sequences of the PCR products from the Tazume strain were genetically very close to the Hirano strain and the Yoshimura strain was also very close to the Karp strain. Furthermore, the DNA sequences from the Irie and Tazume strains were completely homologous to the reported sequences of the Kawasaki and Kuroki strains, respectively.