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Genetic Control of Immune Responses to HIV‐1 env DNA Vaccine
Author(s) -
Ishii Norihisa,
Sugita Yasuyuki,
Nakajima Hiroshi,
Bukawa Hiroki,
Asakura Yusuke,
Okuda Kenji
Publication year - 1997
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1997.tb01873.x
Subject(s) - biology , dna vaccination , antibody , virology , immune system , cd8 , immunology , monoclonal antibody , antigen , immunoglobulin g , antibody response , immunization
We investigated the genetic control of immunoglobulin production and the delayed‐type hypersensitivity (DTH) response produced by an HIV‐specific DNA vaccine using several strains of mice. Murine antigen‐specific immunoglobulin production was determined by ELISA. The DTH response was assessed in terms of the footpad swelling reaction. All strains of mice, except for B10.RIII and B10.T(6R), exhibited strong immunoglobulin production and footpad swelling in response to the DNA vaccine. In vitro treatment of lymphoid cells with monoclonal antibodies showed that the footpad swelling response was mediated by CD4 + 8 − and Ia— T cells. However, CD8 + T cells did not suppress footpad swelling. There was no difference in the induction of HIV‐specific immunoglobulin production or DTH response induced by the DNA vaccine among the strains, suggesting that HIV‐specific DNA vaccine is useful for immunizing various populations against HIV‐1.