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Identification of Murine T Cells Reactive with the Bacterial Superantigen Yersinia pseudotuberculosis‐ Derived Mitogen (YPM) and Factors Involved in YPM‐Induced Toxicity in Mice
Author(s) -
MiyoshiAkiyama Tohru,
Fujimaki Wakae,
Yan XaoJie,
Yagi Junji,
Imanishi Ken'ichi,
Kato Hidehito,
Tomonari Kyuhei,
Uchiyama Takehiko
Publication year - 1997
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1997.tb01211.x
Subject(s) - superantigen , yersinia pseudotuberculosis , t cell receptor , yersiniosis , immunology , cd8 , t cell , yersinia enterocolitica , toxic shock syndrome , microbiology and biotechnology , biology , medicine , antigen , immune system , enterobacteriaceae , staphylococcus aureus , escherichia coli , bacteria , genetics , biochemistry , virulence , gene
We previously reported that Yersinia pseudotuberculosis ‐derived mitogen (YPM) acts as a superantigen to human T cells. In this study, we assessed the superantigenicity and toxicity of YPM using murine experimental models. YPM activated T cells to produce interleukin‐2 in a major histocompatibility complex class II molecule‐dependent manner. The T‐cell blasts induced by YPM expressed T‐cell receptor (TCR) β‐chain variable region (Vβ)7, VβS.1, Vβ8.2 and Vβ8.3. The injection of YPM into mice pre‐sensitized with D‐galactosamine induced lethal shock. This shock was blocked by the injection of monoclonal antibodies (mAbs) to CD4, TCR Vβ7 plus Vβ8, tumor necrosis factor‐α (TNF‐α) and interferon‐γ (IFN‐γ), but not by injection to CD8 or unrelated Vβ. These results indicate that YPM‐induced shock requires the presence of CD4 + T cells bearing TCR Vβ7 and Vβ8, and that endogenous TNF‐a and IFN‐γ mediate the lethal effects.