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Normal Human Fibroblasts Immortalized by Introduction of Human Papillomavirus Type 16 (HPV‐16) E6‐E7 Genes
Author(s) -
Shiga Takashi,
Shirasawa Hiroshi,
Shimizu Kumiko,
Dezawa Mari,
Masuda Yoshiaki,
Simizu Bunsiti
Publication year - 1997
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1997.tb01207.x
Subject(s) - plasmid , biology , cell culture , gene , microbiology and biotechnology , transfection , telomerase , human papillomavirus , doubling time , population , virology , genetics , medicine , demography , sociology
This report demonstrates that normal human fibroblasts can be immortalized by the introduction of HPV‐16 E6‐E7 genes. We designed zinc‐inducible expression plasmids with HPV‐16 E6, E7 or both. Each plasmid was introduced into normal human fibroblasts (TIG‐3 cells) using lipofection methods. Only trans‐fectants with the HPV‐16 E6‐E7 zinc‐inducible expression plasmid, which were cultured in medium supplemented with 100 μM ZnSO 4 , overcame crisis and could be cultured over 200 population doubling levels (PDLs). These cell lines showed the reactivation of telomerase after crisis, and morphological alterations were also observed.