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Infection of an HIV‐1/SIVmac Chimeric Virus Having HIV‐1 env to Macaque Monkeys via the Vaginal Cavity
Author(s) -
Ido Eiji,
Okada Mineyuki,
Enose Yoshimi,
Kuwata Takeo,
Igarashi Tatsuhiko,
Sata Tetsutaro,
Terao Keiji,
Chen Jiangli,
Hayami Masanori
Publication year - 1997
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1997.tb01201.x
Subject(s) - virology , virus , macaque , biology , rhesus macaque , simian immunodeficiency virus , peripheral blood mononuclear cell , antibody , vagina , inoculation , immunology , in vitro , paleontology , biochemistry , genetics
We previously reported that an HIV‐1/SIVmac chimeric virus (designated as NM‐3rN) having HIV‐1 env efficiently infected macaque monkeys by intravenous inoculation. In this study, this chimeric virus was atraumatically inoculated into the vaginal cavity of two rhesus and one cynomolgus monkeys. Although antibody response and detection of proviral genome by PCR were observed in both rhesus monkeys, virus recovery was only once from PBMC in one of them. In the cynomolgus monkey, no virus was recovered and proviral DNA detection was rare. Thus, vaginal inoculation with NM‐3rN resulted in poor systemic infection, implying the presence of selective pressure while passing through mucosal membranes.