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Role of Platelet‐Actlvatlng‐Factor (PAF) on Cellular Responses after Stimulation with Leptospire Lipopolysaccharide
Author(s) -
Isogai Emiko,
Hirose Kimiharu,
Kimura Koichi,
Hayashi Shunji,
Kubota Toru,
Fujii Nobuhiro,
Isogai Hiroshi
Publication year - 1997
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1997.tb01200.x
Subject(s) - lipopolysaccharide , platelet activating factor , platelet , stimulation , activator (genetics) , antagonist , umbilical vein , platelet activation , immunology , pharmacology , tissue factor , endocrinology , biology , medicine , receptor , biochemistry , in vitro , coagulation
Leptospire lipopolysaccharide (LPS) stimulated the adherence of polymorphonuclear neutrophils (PMNs) to human umbilical vein endothelial cells (HUVEC). Enhanced PMN adherence in response to leptospire LPS can be mediated by platelet‐activator‐factor (PAF), because a PAF antagonist reduced adherence. Leptospire LPS also induced the adherence platelets or U937. The second experiment involved leptospire LPS elicited platelet aggregation in a PMN‐platelet mixture, because leptospire LPS stimulated human PMN but not the human platelets. The platelet response was observed only in the mixture system and was inhibited by a PAF antagonist. PAF could be an important pathogenic factor in human leptospirosis.