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Double‐Negative BV8S3 T Cells Expanded in MRL lpr/lpr Lymph Nodes Conserve TCRBJ Gene Usage of Single‐Positive BV8S3 T Cells
Author(s) -
Fan Jiong,
Nanki Toshihiro,
Miyasaka Nobuyuki,
Kohsaka Hitoshi
Publication year - 1997
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1997.tb01197.x
Subject(s) - lymph , biology , gene , double negative , microbiology and biotechnology , genetics , immunology , t cell , pathology , immune system , medicine
Enlarged lymph nodes of mice with lpr mutation consist predominantly of CD4 − CD8 − (double‐negative: DN) T cells. Among them, TCRBV8S3 (Vβ 8.3) T cells are overrepresented as compared to those in single‐positive (SP) T cells. To address the question of whether the expansion of oligoclonal T cells is responsible for the increase in TCRBV8S3 cells, we examined the TCRBJ gene repertoires of BV8S3 DN and SP T cells from multiple MRL lpr/lpr mice. The BJ repertoires of BV3 (Vβ3), BV8S1 (Vβ8.1) and BV8S2 (Vβ8.2) were studied for comparison with those of BV8S3 T cells. The employed method, which was based on a PCR‐ELISA technique, was newly developed and allowed us to make a precise quantitation of TCRBJ gene usage of the multiple lymphocyte samples. The results showed that there were no biases of the BJ gene usage by BV8S3 DN T cells as well as other BV T cells. Furthermore, the BJ gene usage of CD4 and CD8 BV8S3 T cells was conserved by the DN T cells. It is suggested that the BV8S3 DN T cells were not expanded by specific antigens. The expansion may result from aberrant regulation specific to the BV8S3‐expressing T cells.