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Accelerated Progression of a Murine Retrovirus‐Induced Immunodeficiency Syndrome In Fas Mutant C57BL/6/ lpr/lpr Mice
Author(s) -
Hiromatsu Kenji,
Usami Jun,
Aoki Yoshiyasu,
Makino Masahiko,
Yoshikai Yasunobu
Publication year - 1997
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1997.tb01194.x
Subject(s) - retrovirus , murine leukemia virus , cd8 , immunology , microbiology and biotechnology , t cell receptor , virology , immunodeficiency , biology , virus , medicine , antigen , t cell , immune system
We reported previously that CD4 + T cells and B cells in mice with retrovirus‐induced murine acquired immunodeficiency syndrome (MAIDS) caused by LP‐BM5 murine leukemia virus (MuLV) mixtures increased the expression of Fas antigen (Fas) during progression of the disease. However, the contribution of the Fas/Fas ligand (Fas L) system to the pathogenesis of MAIDS remained unknown. Here, we examined the susceptibility of C57BL/6 (B6) lpr/lpr mice, which has been reported to be defective for the expression of Fas, to MAIDS. We found that the Thy 1.2 − CD4 T cells and IgK dull B220 + cells, which are characteristic of MAIDS, increased after the inoculation of LP‐BM5 MuLV in B6 lpr/lpr mice. B22 + TCR αβ T cells, unique to lupus prone mice, also increased in the B6 lpr/lpr mice after infection. CD4 + B220 + TCR αβ T cells increased profoundly among the B220 + TCR αβ T cells from LP‐BM5 MuLV‐infected B6 lpr/lpr mice, while the B220 + TCR αβ T cells observed in non‐infected B6 lpr/lpr mice were largely of the CD4 − CD8 − phenotype. A DNA PCR analysis of the LP‐BM5 MuLV‐infected B6 lpr/lpr mice revealed the genome integration of defective LP‐BM5 virus, further confirming that MAIDS is inducible to B6 lpr/lpr mice. LP‐BM5 MuLV‐infected lpr/lpr mice died within 3 months, while MAIDS‐infected B6 +/+ mice usually died within 5 to 6 months, and B6 lpr/lpr mice not infected with LP‐BM5 MuLV lived more than 6 months. Taken together, these results suggest that MAIDS is inducible independently with functional Fas expression and the possibility of accelerated progression of murine AIDS and lpr ‐associated autoimmune disease in B6 lpr/lpr mice infected with LP‐BM5 MuLV.