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In Vitro Selection of Variants of Herpes Simplex Virus Type 1 Which Differ in Cytopathic Changes
Author(s) -
Padilla Jorge,
Yamada Masao,
Takahashi Yasushi,
Tsukazaki Takashi,
Nakamura Jun,
Yoshida Mariko,
Uno Fumio,
Arao Yujiro,
Nii Shiro
Publication year - 1997
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1997.tb01191.x
Subject(s) - biology , herpes simplex virus , virology , vero cell , cytopathic effect , in vitro , virus , strain (injury) , cell culture , phenotype , interferon , genetics , gene , anatomy
To analyze the mechanisms for in vitro emergence of the syncytial variants of herpes simplex virus type 1 (HSV‐1), several cell lines were infected with a mixture of equal amounts of two HSV‐1 variants, one syncytial and the other non‐syncytial, and changes in their relative abundance were monitored during passage. With a combination of two variants of the Miyama strain of HSV‐1, the syncytial variant became dominant during passage in Vero, RK‐13 and FL cells. On the other hand, the ratios of the two variants remained around 1:1 during the passage in HEp‐2, MGC and HEL cells. In another set of variants of the SKO strain of HSV‐1, the outcomes were different from those of the Miyama strain in the FL, MGC and HEp‐2 cells. The ratios of the two variants remained around 1:1 during passage in FL cells, while the non‐syncytial variant became dominant during passage in MGC and HEp‐2 cells. In addition, we examined the effects of a complement and interferon‐β (IFN‐β) on the outcome of the selection. As a result, the complement slowed the selection of a syncytial variant, whereas IFN‐β facilitated it.