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Levels of Porphyromonas gingivalis Fimbriae and Inflammatory Cytokines in Gingival Crevicular Fluid from Adult Human Subjects
Author(s) -
Hirose Kimiharu,
Isogai Emiko,
Miura Hiroko,
Ueda Itsuo
Publication year - 1997
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1997.tb01168.x
Subject(s) - porphyromonas gingivalis , fimbria , bacteroidaceae , monoclonal antibody , tumor necrosis factor alpha , microbiology and biotechnology , biology , interleukin , cytokine , periodontitis , antibody , immunology , medicine , bacteria , escherichia coli , biochemistry , genetics , gene
The Porphyromonas gingivalis fimbriae level was examined in the gingival crevicular fluid (GCF) from adult human subjects using an immunoblot assay with a monoclonal antibody. The cytokines, interleukin‐1α (IL‐1α), IL‐1β, IL‐6 and tumor necrosis factor‐a (TNF‐α) levels in the GCF were quantified by enzyme‐linked immunosorbent assay (ELISA). The reactivity of the GCF samples with the monoclonal antibody against P. gingivalis fimbriae was related to the IL‐1β, IL‐6 and TNF‐α levels. Moreover, the fimbriae content was associated with the gingival index (GI). In contrast, no significant correlation was seen between the fimbriae content and IL‐1α level. These results suggest that there are possible associations between P. gingivalis fimbriae and IL‐1β, IL‐6 and TNF‐α in the gingival crevicular fluid.