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Analysis of Vibrio cholerae O139 Bengal Isolated from Different Geographical Areas Using Macrorestriction DNA Analysis
Author(s) -
Kurazono Hisao,
Yamasaki Shinji,
Ratchtrachenchai Ornag,
Nair G. Balakrish,
Takeda Yoshifumi
Publication year - 1996
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1996.tb03350.x
Subject(s) - vibrio cholerae , biology , pulsed field gel electrophoresis , genomic dna , microbiology and biotechnology , genetics , dna profiling , dna , genotype , vibrionaceae , clone (java method) , gel electrophoresis , gene , bacteria
Vibrio cholerae O139 isolated from different countries, as well as from different locations within a country, were examined using macrorestriction DNA analysis to determine the clonality of the O139 strains. Not I digests of genomic DNA of representative strains from Nepal, India, Bangladesh, China, Thailand, and Malaysia revealed very similar but not identical patterns. Examinations of the banding patterns generated by pulsed‐field gel electrophoresis of strains isolated within countries revealed complete homogeneity. These results further reiterate the spread of an identical clone of V. cholerae O139 although it appears that genetic polymorphism among the O139 strains is becoming apparent.