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Evidence That Two Distinct Populations of Rabbit Anti‐Idiotypic Antibodies Are Induced by Three Monoclonal Antibodies Specific for Bluetongue Virus Core Protein VP7
Author(s) -
Lin Min,
Zhou EnMin
Publication year - 1996
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1996.tb03349.x
Subject(s) - epitope , monoclonal antibody , antibody , antigen , antiserum , idiotype , virology , medicine , biology , immunology
Three groups of anti‐idiotypic antibodies (anti‐Id or Ab2), designated RAb2‐A, RAb2‐B1, and RAb2‐B2, were isolated from rabbit antiserum raised against three monoclonal antibodies (mAbs) (M1875, M1877, and M1886) specific for the bluetongue virus core protein, VP7. RAb2‐A was specific for the idiotype of M1875. RAb2‐B1 and RAb2‐B2, isolated through the M1877 and M1886 affinity columns, respectively, were directed against the common idiotype that is shared by M1877 and M1886 and therefore classified in the same population (RAb2‐B). Further characterization revealed that the two anti‐Id populations, RAb2‐A and RAb2‐B, were significantly different. RAb2‐A was an Ab2β type of anti‐Id since (i) its reaction with M1875 was inhibited by the antigen; (ii) it inhibited the M1875‐VP7 interaction; and (iii) it elicited anti‐VP7 antibody response in Balb/c mice. In contrast, RAb2‐B may represent an Ab2α type of anti‐Id since its reactions with M1877 or M1886 were not inhibited by the antigen, even though it inhibited mAbs from binding to the antigen. These results indicated that RAb2‐A and RAb2‐B represent two distinct populations of anti‐Ids to anti‐VP7 mAbs with similar epitope specificity.